Canditate metabolites for ash dieback tolerance in Fraxinus excelsior

Miguel Nemesio-Gorriz, Riya C Menezes, Christian Paetz, Almuth Hammerbacher, Marijke Steenackers, Kurt Schamp, Monica Höfte, Ales Svatos, Jonathan Gershenzon, Gerry C Douglas

Research output: Contribution to journalA1: Web of Science-article

Abstract

Ash dieback, a forest epidemic caused by the invasive fungus Hymenoscyphus fraxineus, threatens ash trees throughout Europe. Within Fraxinus excelsior populations, a small proportion of genotypes show a low susceptibility to the pathogen. We compared the metabolomes from a cohort of low-susceptibility ash genotypes with a cohort of high-susceptibility ash genotypes. This revealed two significantly different chemotypes. A total of 64 candidate metabolites associated with reduced or increased susceptibility in the chemical families secoiridoids, coumarins, flavonoids, phenylethanoids, and lignans. Increased levels of two coumarins, fraxetin and esculetin, were strongly associated with reduced susceptibility to ash dieback. Both coumarins inhibited the growth of H. fraxineus in vitro when supplied at physiological concentrations, thereby validating their role as markers for low susceptibility to ash dieback. Similarly, fungal growth inhibition was observed when the methanolic bark extract of low-susceptibility ash genotypes was supplied. Our findings indicate the presence of constitutive chemical defense barriers against ash dieback in ash.
Original languageEnglish
Volume71
Issue number19
Pages (from-to)6074-6083
Number of pages9
ISSN1460-2431
DOIs
Publication statusPublished - 7-Oct-2020

Thematic List 2020

  • Forest

Taxonomic list

  • ash (Fraxinus excelsior)

Policy

  • woodland protection

Geographic list

  • Europe

Technological

  • genetic technologies
  • phenotyping

Free keywords

  • ash dieback
  • Hymenoscyphus fraxineus
  • metabolites

Fingerprint Dive into the research topics of 'Canditate metabolites for ash dieback tolerance in Fraxinus excelsior'. Together they form a unique fingerprint.

Cite this