Fine Mapping and Identification of Nucleotide Binding Site/Leucine-Rich Repeat Sequences at the MER Locus in Populus deltoides 'S9-2'

J Zhang; M Steenackers; V Storme; S Neyrinck; M Van Montagu; T Gerats; W Boerjan

doi:10.1094/PHYTO.2001.91.11.1069

Fine Mapping and Identification of Nucleotide Binding Site/Leucine-Rich Repeat Sequences at the MER Locus in Populus deltoides 'S9-2'

J Zhang, M Steenackers, V Storme, S Neyrinck, M Van Montagu, T Gerats, W Boerjan

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Melampsora larici-populina is the most damaging leaf pathogen for poplar in Europe. Previous genetic analyses have revealed both qualitative and quantitative resistance to this fungus. As a starting point for positional cloning of the gene or genes conferring qualitative resistance to M. larici-populina races E1, E2, and E3, a local genetic map of the Melampsora resistance (MER) locus was constructed based on amplified fragment length polymorphism (AFLP) markers. Eleven AFLP markers were identified by bulked segregant analysis. These markers were used to identify 17 recombinants at the MER locus, from a total of 512 progenies derived from three interspecific crosses involving the same resistant female parent, Populus deltoides 'S9-2'. The local genetic map covered a 3.4-centimorgan interval encompassing the target locus. Sequence analysis of these AFLP markers revealed similarities to the nucleotide binding site/leucine-rich repeat class of disease resistance genes.

Original language	English
Journal	Phytopathology
Volume	91
Issue number	11
Pages (from-to)	1069-73
Number of pages	5
ISSN	0031-949X
DOIs	https://doi.org/10.1094/PHYTO.2001.91.11.1069
Publication status	Published - 2001

Taxonomic list

poplar (Populus spp.)

Technological

genetic technologies

Free keywords

mapping
disease resistance

Access to Document

10.1094/PHYTO.2001.91.11.1069

1 A1: Web of Science-article

Field and saccharification performances of poplars severely downregulated in CAD1
De Meester, B., Van Acker, R., Wouters, M., Traversari, S., Steenackers, M., Neukermans, J., Van Breusegem, F., Dejardin, A., Pilate, G. & Boerjan, W., 8-Jul-2022, (E-pub ahead of print) In: New Phytologist. doi: 10.1111/nph.18366
Research output: Contribution to journal › A1: Web of Science-article › peer-review

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@article{890b58fbaa92425b96c038e1ae50c20a,

title = "Fine Mapping and Identification of Nucleotide Binding Site/Leucine-Rich Repeat Sequences at the MER Locus in Populus deltoides 'S9-2'",

abstract = "Melampsora larici-populina is the most damaging leaf pathogen for poplar in Europe. Previous genetic analyses have revealed both qualitative and quantitative resistance to this fungus. As a starting point for positional cloning of the gene or genes conferring qualitative resistance to M. larici-populina races E1, E2, and E3, a local genetic map of the Melampsora resistance (MER) locus was constructed based on amplified fragment length polymorphism (AFLP) markers. Eleven AFLP markers were identified by bulked segregant analysis. These markers were used to identify 17 recombinants at the MER locus, from a total of 512 progenies derived from three interspecific crosses involving the same resistant female parent, Populus deltoides 'S9-2'. The local genetic map covered a 3.4-centimorgan interval encompassing the target locus. Sequence analysis of these AFLP markers revealed similarities to the nucleotide binding site/leucine-rich repeat class of disease resistance genes.",

author = "J Zhang and M Steenackers and V Storme and S Neyrinck and {Van Montagu}, M and T Gerats and W Boerjan",

year = "2001",

doi = "10.1094/PHYTO.2001.91.11.1069",

language = "English",

volume = "91",

pages = "1069--73",

journal = "Phytopathology",

issn = "0031-949X",

publisher = "American Phytopathological Society",

number = "11",

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T1 - Fine Mapping and Identification of Nucleotide Binding Site/Leucine-Rich Repeat Sequences at the MER Locus in Populus deltoides 'S9-2'

AU - Zhang, J

AU - Steenackers, M

AU - Storme, V

AU - Neyrinck, S

AU - Van Montagu, M

AU - Gerats, T

AU - Boerjan, W

PY - 2001

Y1 - 2001

N2 - Melampsora larici-populina is the most damaging leaf pathogen for poplar in Europe. Previous genetic analyses have revealed both qualitative and quantitative resistance to this fungus. As a starting point for positional cloning of the gene or genes conferring qualitative resistance to M. larici-populina races E1, E2, and E3, a local genetic map of the Melampsora resistance (MER) locus was constructed based on amplified fragment length polymorphism (AFLP) markers. Eleven AFLP markers were identified by bulked segregant analysis. These markers were used to identify 17 recombinants at the MER locus, from a total of 512 progenies derived from three interspecific crosses involving the same resistant female parent, Populus deltoides 'S9-2'. The local genetic map covered a 3.4-centimorgan interval encompassing the target locus. Sequence analysis of these AFLP markers revealed similarities to the nucleotide binding site/leucine-rich repeat class of disease resistance genes.

AB - Melampsora larici-populina is the most damaging leaf pathogen for poplar in Europe. Previous genetic analyses have revealed both qualitative and quantitative resistance to this fungus. As a starting point for positional cloning of the gene or genes conferring qualitative resistance to M. larici-populina races E1, E2, and E3, a local genetic map of the Melampsora resistance (MER) locus was constructed based on amplified fragment length polymorphism (AFLP) markers. Eleven AFLP markers were identified by bulked segregant analysis. These markers were used to identify 17 recombinants at the MER locus, from a total of 512 progenies derived from three interspecific crosses involving the same resistant female parent, Populus deltoides 'S9-2'. The local genetic map covered a 3.4-centimorgan interval encompassing the target locus. Sequence analysis of these AFLP markers revealed similarities to the nucleotide binding site/leucine-rich repeat class of disease resistance genes.

U2 - 10.1094/PHYTO.2001.91.11.1069

DO - 10.1094/PHYTO.2001.91.11.1069

M3 - A1: Web of Science-article

C2 - 18943442

SN - 0031-949X

VL - 91

SP - 1069

EP - 1073

JO - Phytopathology

JF - Phytopathology

IS - 11

ER -

Fine Mapping and Identification of Nucleotide Binding Site/Leucine-Rich Repeat Sequences at the MER Locus in Populus deltoides 'S9-2'

Abstract

Taxonomic list

Technological

Free keywords

Access to Document

Fingerprint

Research output

Field and saccharification performances of poplars severely downregulated in CAD1

Cite this