Isolation, characterization and genotyping of single nucleotide polymorphisms in the non-model tree species Frangula alnus (Rhamnaceae)

Hanne De Kort, Katrien Vandepitte, Joachim Mergeay, Olivier Honnay

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    Abstract

    The genomic content of the non-model plant Frangula alnus remains largely unexplored. However, because of its occurrence over a wide latitudinal range and its invasive nature in the US and Canada, F. alnus may serve as an excellent study species in tree conservation genomic research. We used pooled paired-end sequencing of Restriction-site Associated DNA to discover SNPs in the insect pollinated shrub F. alnus. We identified 7,383 SNPs from which the corresponding contig sequences were annotated to the available plant genomes. An enrichment analysis showed an excess of putatively adaptive and plastic gene ontology terms.
    Original languageEnglish
    JournalConservation Genetics Resources
    Volume6
    Pages (from-to)267-269
    Number of pages3
    DOIs
    Publication statusPublished - 2014

    Thematic list

    • Species and biotopes

    EWI Biomedical sciences

    • B003-ecology

    Policy

    • forest and agriculture policy

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