Isolation, characterization and genotyping of single nucleotide polymorphisms in the non-model tree species Frangula alnus (Rhamnaceae)

The genomic content of the non-model plant Frangula alnus remains largely unexplored. However, because of its occurrence over a wide latitudinal range and its invasive nature in the US and Canada, F. alnus may serve as an excellent study species in tree conservation genomic research. We used pooled paired-end sequencing of Restriction-site Associated DNA to discover SNPs in the insect pollinated shrub F. alnus. We identified 7,383 SNPs from which the corresponding contig sequences were annotated to the available plant genomes. An enrichment analysis showed an excess of putatively adaptive and plastic gene ontology terms.