Genome-wide methylome stability and parental effects in the worldwide distributed Lombardy poplar

Methylation profiling by high throughput sequencing of 29 Lombardy poplar (Populus nigra cv. 'Italica' Duroi.) samples on the Illumina NovaSeq 6000 platform.

Data Public on Feb 20, 2023
BioProject: PRJNA936584
GEO DataSet: GEO: GSE225596

We present detailed methylome analyses of the long-lived Lombardy poplar, Populus nigra cv. ‘Italica‘ Duroi. Trees of this widespread clonal cultivar descended from a common ancestor at least two centuries ago.

We analyzed genome-wide methylomes of in total 13 pooled and 16 individual DNA-samples. We found that ramets collected over Europe and raised in common conditions have stable methylomes in symmetrical CG-contexts over at least two years and across vegetatively produced generations. In contrast, seasonal dynamics occurred in methylation patterns in CHH-context.

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