Fine Mapping and Identification of Nucleotide Binding Site/Leucine-Rich Repeat Sequences at the MER Locus in Populus deltoides 'S9-2'

J Zhang; M Steenackers; V Storme; S Neyrinck; M Van Montagu; T Gerats; W Boerjan

doi:10.1094/PHYTO.2001.91.11.1069

Fine Mapping and Identification of Nucleotide Binding Site/Leucine-Rich Repeat Sequences at the MER Locus in Populus deltoides 'S9-2'

J Zhang, M Steenackers, V Storme, S Neyrinck, M Van Montagu, T Gerats, W Boerjan

Onderzoeksoutput: Bijdrage aan tijdschrift › A1: Web of Science-artikel › peer review

Uittreksel

Melampsora larici-populina is the most damaging leaf pathogen for poplar in Europe. Previous genetic analyses have revealed both qualitative and quantitative resistance to this fungus. As a starting point for positional cloning of the gene or genes conferring qualitative resistance to M. larici-populina races E1, E2, and E3, a local genetic map of the Melampsora resistance (MER) locus was constructed based on amplified fragment length polymorphism (AFLP) markers. Eleven AFLP markers were identified by bulked segregant analysis. These markers were used to identify 17 recombinants at the MER locus, from a total of 512 progenies derived from three interspecific crosses involving the same resistant female parent, Populus deltoides 'S9-2'. The local genetic map covered a 3.4-centimorgan interval encompassing the target locus. Sequence analysis of these AFLP markers revealed similarities to the nucleotide binding site/leucine-rich repeat class of disease resistance genes.

Oorspronkelijke taal	Engels
Tijdschrift	Phytopathology
Volume	91
Exemplaarnummer	11
Pagina's (van-tot)	1069-73
Aantal pagina’s	5
ISSN	0031-949X
DOI's	https://doi.org/10.1094/PHYTO.2001.91.11.1069
Publicatiestatus	Gepubliceerd - 2001

Thematische lijst

Soorten en biotopen

EWI Biomedische wetenschappen

B004-plantkunde

Taxonomische lijst

populier (Populus spp.)

Technologisch

genetische technieken

Toegang verkrijgen tot document

10.1094/PHYTO.2001.91.11.1069

1 A1: Web of Science-artikel

Field and saccharification performances of poplars severely downregulated in CAD1
De Meester, B., Van Acker, R., Wouters, M., Traversari, S., Steenackers, M., Neukermans, J., Van Breusegem, F., Dejardin, A., Pilate, G. & Boerjan, W., 8-jul.-2022, (E-publicatie voorafgaand op geprinte versie) In: New Phytologist. doi: 10.1111/nph.18366
Onderzoeksoutput: Bijdrage aan tijdschrift › A1: Web of Science-artikel › peer review

7 Downloads (Pure)

Dit citeren

@article{890b58fbaa92425b96c038e1ae50c20a,

title = "Fine Mapping and Identification of Nucleotide Binding Site/Leucine-Rich Repeat Sequences at the MER Locus in Populus deltoides 'S9-2'",

abstract = "Melampsora larici-populina is the most damaging leaf pathogen for poplar in Europe. Previous genetic analyses have revealed both qualitative and quantitative resistance to this fungus. As a starting point for positional cloning of the gene or genes conferring qualitative resistance to M. larici-populina races E1, E2, and E3, a local genetic map of the Melampsora resistance (MER) locus was constructed based on amplified fragment length polymorphism (AFLP) markers. Eleven AFLP markers were identified by bulked segregant analysis. These markers were used to identify 17 recombinants at the MER locus, from a total of 512 progenies derived from three interspecific crosses involving the same resistant female parent, Populus deltoides 'S9-2'. The local genetic map covered a 3.4-centimorgan interval encompassing the target locus. Sequence analysis of these AFLP markers revealed similarities to the nucleotide binding site/leucine-rich repeat class of disease resistance genes.",

author = "J Zhang and M Steenackers and V Storme and S Neyrinck and {Van Montagu}, M and T Gerats and W Boerjan",

year = "2001",

doi = "10.1094/PHYTO.2001.91.11.1069",

language = "English",

volume = "91",

pages = "1069--73",

journal = "Phytopathology",

issn = "0031-949X",

publisher = "American Phytopathological Society",

number = "11",

}

TY - JOUR

T1 - Fine Mapping and Identification of Nucleotide Binding Site/Leucine-Rich Repeat Sequences at the MER Locus in Populus deltoides 'S9-2'

AU - Zhang, J

AU - Steenackers, M

AU - Storme, V

AU - Neyrinck, S

AU - Van Montagu, M

AU - Gerats, T

AU - Boerjan, W

PY - 2001

Y1 - 2001

N2 - Melampsora larici-populina is the most damaging leaf pathogen for poplar in Europe. Previous genetic analyses have revealed both qualitative and quantitative resistance to this fungus. As a starting point for positional cloning of the gene or genes conferring qualitative resistance to M. larici-populina races E1, E2, and E3, a local genetic map of the Melampsora resistance (MER) locus was constructed based on amplified fragment length polymorphism (AFLP) markers. Eleven AFLP markers were identified by bulked segregant analysis. These markers were used to identify 17 recombinants at the MER locus, from a total of 512 progenies derived from three interspecific crosses involving the same resistant female parent, Populus deltoides 'S9-2'. The local genetic map covered a 3.4-centimorgan interval encompassing the target locus. Sequence analysis of these AFLP markers revealed similarities to the nucleotide binding site/leucine-rich repeat class of disease resistance genes.

AB - Melampsora larici-populina is the most damaging leaf pathogen for poplar in Europe. Previous genetic analyses have revealed both qualitative and quantitative resistance to this fungus. As a starting point for positional cloning of the gene or genes conferring qualitative resistance to M. larici-populina races E1, E2, and E3, a local genetic map of the Melampsora resistance (MER) locus was constructed based on amplified fragment length polymorphism (AFLP) markers. Eleven AFLP markers were identified by bulked segregant analysis. These markers were used to identify 17 recombinants at the MER locus, from a total of 512 progenies derived from three interspecific crosses involving the same resistant female parent, Populus deltoides 'S9-2'. The local genetic map covered a 3.4-centimorgan interval encompassing the target locus. Sequence analysis of these AFLP markers revealed similarities to the nucleotide binding site/leucine-rich repeat class of disease resistance genes.

U2 - 10.1094/PHYTO.2001.91.11.1069

DO - 10.1094/PHYTO.2001.91.11.1069

M3 - A1: Web of Science-article

C2 - 18943442

SN - 0031-949X

VL - 91

SP - 1069

EP - 1073

JO - Phytopathology

JF - Phytopathology

IS - 11

ER -

Fine Mapping and Identification of Nucleotide Binding Site/Leucine-Rich Repeat Sequences at the MER Locus in Populus deltoides 'S9-2'

Uittreksel

Thematische lijst

EWI Biomedische wetenschappen

Taxonomische lijst

Technologisch

Toegang verkrijgen tot document

Vingerafdruk

Onderzoeksoutput

Field and saccharification performances of poplars severely downregulated in CAD1

Dit citeren