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Ex-situ conservation of black poplar in Europe: genetic diversity in nine gene bank collections and their value for nature development

Research output: Contribution to journalA1: Web of Science-article

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Ex-situ conservation of black poplar in Europe : genetic diversity in nine gene bank collections and their value for nature development. / Storme, V; Vanden Broeck, An; Ivens, B; Halfmaerten, David; Van Slycken, Jos; Castiglione, S; Grassi, F; Fossati, T; Cottrell, J. E; Tabbener, H. E; Lefevre, F; Saintagne, C; Fluch, S; Krystufek, V; Burg, K; Bordacs, S; Borovics, A; Gebhardt, K; Vornam, B; Pohl, A; Alba, N; Agundez, D; Maestro, C; Notivol, E; Bovenschen, J; van Dam, B. C; van der Schoot, J; Vosman, B; Boerjan, W; Smulders, M. J. M.

In: Theoretical and applied genetics, Vol. 108, 2004, p. 969-981.

Research output: Contribution to journalA1: Web of Science-article

Harvard

Storme, V, Vanden Broeck, A, Ivens, B, Halfmaerten, D, Van Slycken, J, Castiglione, S, Grassi, F, Fossati, T, Cottrell, JE, Tabbener, HE, Lefevre, F, Saintagne, C, Fluch, S, Krystufek, V, Burg, K, Bordacs, S, Borovics, A, Gebhardt, K, Vornam, B, Pohl, A, Alba, N, Agundez, D, Maestro, C, Notivol, E, Bovenschen, J, van Dam, BC, van der Schoot, J, Vosman, B, Boerjan, W & Smulders, MJM 2004, 'Ex-situ conservation of black poplar in Europe: genetic diversity in nine gene bank collections and their value for nature development', Theoretical and applied genetics, vol. 108, pp. 969-981. https://doi.org/10.1007/s00122-003-1523-6

APA

Author

Storme, V ; Vanden Broeck, An ; Ivens, B ; Halfmaerten, David ; Van Slycken, Jos ; Castiglione, S ; Grassi, F ; Fossati, T ; Cottrell, J. E ; Tabbener, H. E ; Lefevre, F ; Saintagne, C ; Fluch, S ; Krystufek, V ; Burg, K ; Bordacs, S ; Borovics, A ; Gebhardt, K ; Vornam, B ; Pohl, A ; Alba, N ; Agundez, D ; Maestro, C ; Notivol, E ; Bovenschen, J ; van Dam, B. C ; van der Schoot, J ; Vosman, B ; Boerjan, W ; Smulders, M. J. M. / Ex-situ conservation of black poplar in Europe : genetic diversity in nine gene bank collections and their value for nature development. In: Theoretical and applied genetics. 2004 ; Vol. 108. pp. 969-981.

Bibtex

@article{85f4ac7e320541e7a57b17277bdca25f,
title = "Ex-situ conservation of black poplar in Europe: genetic diversity in nine gene bank collections and their value for nature development",
abstract = "Populus nigra L. is a pioneer tree species of riparian ecosystems that is threatened with extinction because of the loss of its natural habitat. To evaluate the existing genetic diversity of P. nigra within ex-situ collections, we analyzed 675 P. nigra L. accessions from nine European gene banks with three amplified fragment length polymorphism (AFLP) and five microsatellite [or simple sequence repeat (SSR)] primer combinations, and 11 isozyme systems. With isozyme analysis, hybrids could be detected, and only 3{\%} were found in the gene bank collection. AFLP and SSR analyses revealed effectively that 26{\%} of the accessions were duplicated and that the level of clonal duplication varied from 0{\%} in the French gene bank collection up to 78{\%} in the Belgian gene bank collection. SSR analysis was preferred because AFLP was technically more demanding and more prone to scoring errors. To assess the genetic diversity, we grouped material from the gene banks according to topography of the location from which the accessions were originally collected (river system or regions separated by mountains). Genetic diversity was expressed in terms of the following parameters: percentage of polymorphic loci, observed and effective number of alleles, and Neis expected heterozygosity or gene diversity (for AFLP). Genetic diversity varied from region to region and depended, to some extent, on the marker system used. The most unique alleles were identified in the Danube region (Austria), the Rh{\^o}ne region (France), Italy, the Rijn region (The Netherlands), and the Ebro region (Spain). In general, the diversity was largest in the material collected from the regions in Southern Europe. Dendrograms and principal component analysis resulted in a clustering according to topography. Material from the same river systems, but from different countries, clustered together. The genetic differentiation among the regions (Fst/Gst) was moderate.",
author = "V Storme and {Vanden Broeck}, An and B Ivens and David Halfmaerten and {Van Slycken}, Jos and S Castiglione and F Grassi and T Fossati and Cottrell, {J. E} and Tabbener, {H. E} and F Lefevre and C Saintagne and S Fluch and V Krystufek and K Burg and S Bordacs and A Borovics and K Gebhardt and B Vornam and A Pohl and N Alba and D Agundez and C Maestro and E Notivol and J Bovenschen and {van Dam}, {B. C} and {van der Schoot}, J and B Vosman and W Boerjan and Smulders, {M. J. M}",
year = "2004",
doi = "10.1007/s00122-003-1523-6",
language = "English",
volume = "108",
pages = "969--981",
journal = "Theoretical and applied genetics",
issn = "0040-5752",
publisher = "Springer Verlag",

}

RIS

TY - JOUR

T1 - Ex-situ conservation of black poplar in Europe

T2 - genetic diversity in nine gene bank collections and their value for nature development

AU - Storme, V

AU - Vanden Broeck, An

AU - Ivens, B

AU - Halfmaerten, David

AU - Van Slycken, Jos

AU - Castiglione, S

AU - Grassi, F

AU - Fossati, T

AU - Cottrell, J. E

AU - Tabbener, H. E

AU - Lefevre, F

AU - Saintagne, C

AU - Fluch, S

AU - Krystufek, V

AU - Burg, K

AU - Bordacs, S

AU - Borovics, A

AU - Gebhardt, K

AU - Vornam, B

AU - Pohl, A

AU - Alba, N

AU - Agundez, D

AU - Maestro, C

AU - Notivol, E

AU - Bovenschen, J

AU - van Dam, B. C

AU - van der Schoot, J

AU - Vosman, B

AU - Boerjan, W

AU - Smulders, M. J. M

PY - 2004

Y1 - 2004

N2 - Populus nigra L. is a pioneer tree species of riparian ecosystems that is threatened with extinction because of the loss of its natural habitat. To evaluate the existing genetic diversity of P. nigra within ex-situ collections, we analyzed 675 P. nigra L. accessions from nine European gene banks with three amplified fragment length polymorphism (AFLP) and five microsatellite [or simple sequence repeat (SSR)] primer combinations, and 11 isozyme systems. With isozyme analysis, hybrids could be detected, and only 3% were found in the gene bank collection. AFLP and SSR analyses revealed effectively that 26% of the accessions were duplicated and that the level of clonal duplication varied from 0% in the French gene bank collection up to 78% in the Belgian gene bank collection. SSR analysis was preferred because AFLP was technically more demanding and more prone to scoring errors. To assess the genetic diversity, we grouped material from the gene banks according to topography of the location from which the accessions were originally collected (river system or regions separated by mountains). Genetic diversity was expressed in terms of the following parameters: percentage of polymorphic loci, observed and effective number of alleles, and Neis expected heterozygosity or gene diversity (for AFLP). Genetic diversity varied from region to region and depended, to some extent, on the marker system used. The most unique alleles were identified in the Danube region (Austria), the Rhône region (France), Italy, the Rijn region (The Netherlands), and the Ebro region (Spain). In general, the diversity was largest in the material collected from the regions in Southern Europe. Dendrograms and principal component analysis resulted in a clustering according to topography. Material from the same river systems, but from different countries, clustered together. The genetic differentiation among the regions (Fst/Gst) was moderate.

AB - Populus nigra L. is a pioneer tree species of riparian ecosystems that is threatened with extinction because of the loss of its natural habitat. To evaluate the existing genetic diversity of P. nigra within ex-situ collections, we analyzed 675 P. nigra L. accessions from nine European gene banks with three amplified fragment length polymorphism (AFLP) and five microsatellite [or simple sequence repeat (SSR)] primer combinations, and 11 isozyme systems. With isozyme analysis, hybrids could be detected, and only 3% were found in the gene bank collection. AFLP and SSR analyses revealed effectively that 26% of the accessions were duplicated and that the level of clonal duplication varied from 0% in the French gene bank collection up to 78% in the Belgian gene bank collection. SSR analysis was preferred because AFLP was technically more demanding and more prone to scoring errors. To assess the genetic diversity, we grouped material from the gene banks according to topography of the location from which the accessions were originally collected (river system or regions separated by mountains). Genetic diversity was expressed in terms of the following parameters: percentage of polymorphic loci, observed and effective number of alleles, and Neis expected heterozygosity or gene diversity (for AFLP). Genetic diversity varied from region to region and depended, to some extent, on the marker system used. The most unique alleles were identified in the Danube region (Austria), the Rhône region (France), Italy, the Rijn region (The Netherlands), and the Ebro region (Spain). In general, the diversity was largest in the material collected from the regions in Southern Europe. Dendrograms and principal component analysis resulted in a clustering according to topography. Material from the same river systems, but from different countries, clustered together. The genetic differentiation among the regions (Fst/Gst) was moderate.

U2 - 10.1007/s00122-003-1523-6

DO - 10.1007/s00122-003-1523-6

M3 - A1: Web of Science-article

VL - 108

SP - 969

EP - 981

JO - Theoretical and applied genetics

JF - Theoretical and applied genetics

SN - 0040-5752

ER -

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